| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073582 |
| Chromosome: | chromosome 14 |
| Location: | 458430 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610801 | 5'UTR | ||
| lncRNA_TCONS_00189579 | 5'UTR | ||
| lncRNA_TCONS_00189580 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAGTATTGCCGTCCCGTTAATATTAACGCGTGTAAGCACGCACTTGT |
| Internal bar code: | ACCCTGGTTTCGGCCACGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 3.27869 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGTCTGGCACTTTGTTT |
| Suggested primer 2: | ACGTCGTCCGCTTTGTAGTT |