| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073588 |
| Chromosome: | chromosome 3 |
| Location: | 867068 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146447 | (1 of 1) PTHR23244//PTHR23244:SF253 - KELCH REPEAT DOMAIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACTGCTCCGGGGCTCCAATCAGCCCGCTGACAGTGCTGACGGGCTGT |
| Internal bar code: | TACATAACAGGTATTTGAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 284 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGCATGGGTAATGGTGG |
| Suggested primer 2: | GAACTTTGGCTGATAGCGCG |