Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.073592 |
Chromosome: | chromosome 15 |
Location: | 5331265 |
Confidence (%): | 63 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g734400 | CUL2,CUL3 | (1 of 1) K03347 - cullin 1 (CUL1, CDC53); Ubiquitin ligase SCF complex subunit Cullin | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATACTATGGGGTGACGAGGAGATGTCAGACGGCCAGGGGGGGGGGAGA |
Internal bar code: | CCCTACAGCGGAGTAGAGCTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2684 |
LEAP-Seq percent confirming: | 31.9149 |
LEAP-Seq n confirming: | 15 |
LEAP-Seq n nonconfirming: | 32 |
LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTTGACAAGGCTGCCAATG |
Suggested primer 2: | ACACGAGATAGGGGTCACGA |