Insertion junction: CLIP2.073593_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.073593
Chromosome:plastome
Location:30440
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802279 rps8,2716952 30S ribosomal protein S8; (1 of 1) PTHR11758//PTHR11758:SF17 - 40S RIBOSOMAL PROTEIN S15A // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGAAGGACGCTCGTTAAACTCCTGCAAGCAAGTCAACAGTGTCAACCT
Internal bar code:TTATTTGGACTCACTCACCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:137
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTAGTGGACGTCAGTGGC
Suggested primer 2:CACGTAGACGAGCTTCACGA