Insertion junction: CLIP2.073604_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073604
Chromosome:chromosome 16
Location:2447385
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre16.g801913 (1 of 1) PTHR11700:SF11 - 40S RIBOSOMAL PROTEIN S20 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGCCATGCCATTGCCATTGAGCCACAGGACATGCACGGACTTTCCCAC
Internal bar code:GCGCAAATTACGCTTTGTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:995
LEAP-Seq percent confirming:38.8889
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCAGGAAGAGCAACCAG
Suggested primer 2:CGTCATTCACAGCGCAGAAG