| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073612 |
| Chromosome: | chromosome 13 |
| Location: | 2826936 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAACAGGGGGAAAGGGCAAGGGGCAGGTTCGCGTAGTCCCGCAGTGT |
| Internal bar code: | CTCGTTTTCGACGCGCGATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCAGAGGTCTTTGTGGA |
| Suggested primer 2: | GAGACGCGGGCTAATACTCC |