| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.073613 |
| Chromosome: | plastome |
| Location: | 203893 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802336 | orf854 | Wendy transposon | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAATCAGGATCAAAAAGAAACGGAAAATAGTTATTTTAATAACTTAAC |
| Internal bar code: | TCTCCGCTACGTGCTCGCATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 31.0345 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCAACTTGGCGGCTAA |
| Suggested primer 2: | AATCAGGTGGAAGCTTCCGG |