| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073614 |
| Chromosome: | chromosome 2 |
| Location: | 6967878 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389319 | (1 of 2) PTHR11266:SF17 - PROTEIN MPV17 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCGCCAGCGTGACCAGCGGTGTGTGCGCCGAAGGCGCTGGCAAACAC |
| Internal bar code: | ATGGATGCACCTCTCTCTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3117 |
| LEAP-Seq percent confirming: | 42.2764 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCCACGATTCTGGAAAA |
| Suggested primer 2: | GCGGACACACATAAGAACGC |