| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073615 |
| Chromosome: | chromosome 10 |
| Location: | 6177955 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462700 | (1 of 1) K14397 - cleavage and polyadenylation specificity factor subunit 5 (NUDT21, CPSF5, CFIM25) | 3'UTR | |
| Cre10.g462750 | (1 of 1) K11374 - elongator complex protein 2 (ELP2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCAGGCGTGGTGGCGAGCTGACGACTGTGGCCGGCCCATGACACGGC |
| Internal bar code: | CCTCAGTAGACGGGCGAAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1613 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGGCAAGAGGAGAAAGG |
| Suggested primer 2: | GTTTGACCTGCACGACAACC |