| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073624 |
| Chromosome: | chromosome 17 |
| Location: | 4572862 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731800 | (1 of 2) PTHR10913 - FOLLISTATIN-RELATED | 5'UTR | |
| Cre17.g731850 | SDR29 | Short-chain dehydrogenase/reductase; (1 of 1) 1.3.1.34 - 2,4-dienoyl-CoA reductase (NADPH) / 4-enoyl-CoA reductase (NADPH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCCCAGTTCCCTTTTGCAGCGCTTGACCTTAACACAGCATGCCGTAG |
| Internal bar code: | AGCACAACAGTTCAGACGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2830 |
| LEAP-Seq percent confirming: | 65.3846 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTCACGGTGCAAGTTC |
| Suggested primer 2: | ACCCGATGCAAAAGAGGGAG |