| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073629 |
| Chromosome: | chromosome 13 |
| Location: | 4089254 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591000 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCGGCGGGCGGGGCGGTTGGTGGCGCCAGTGGCAGTGGCAGTGGC |
| Internal bar code: | CTCTGGCTCTTCAAGCAATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 44.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCTATCCCTTTCCGCCA |
| Suggested primer 2: | AGTTGCATGGACTGGGATGG |