Insertion junction: CLIP2.073632_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.073632
Chromosome:chromosome 2
Location:804532
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g078550 FAP210 (1 of 5) PF13868 - Trichohyalin-plectin-homology domain (TPH); Flagellar Associated Protein 210 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAAGAGATCAAGCGCCAGATCGAGGTGCTCAAGCGGGCGCAGGAGCAG
Internal bar code:AATCTGATGAAGTGGGGGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1723
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCACCTCTACTACCCCA
Suggested primer 2:GCGCCTAAACGAATGCAGAG