| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073635 |
| Chromosome: | chromosome 9 |
| Location: | 682217 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404550 | (1 of 2) 2.7.11.20 - [Elongation factor 2] kinase / EF2K | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCTACGGTGCCTTGTTGCGGGACGCGGGCGGACCGCAAGTGGGGCT |
| Internal bar code: | CGGTCTGTAAGGCGAGTAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCAGAGTAAGGGTGGGC |
| Suggested primer 2: | TGGGTGGTTTGGATGCCTAC |