Insertion junction: CLIP2.073642_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.073642
Chromosome:chromosome 6
Location:210842
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g250450 (1 of 5) K14347 - solute carrier family 10 (sodium/bile acid cotransporter), member 7 (SLC10A7, P7); Sodium:bile acid symporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCTCCCCCGGCTTTCTGTGTGAACAATTTGAGTGGTCCACCAGCTCTC
Internal bar code:GTCCAGACGCGGGTTATCGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4475
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCAGAATCATGGTCACCA
Suggested primer 2:TGCGTTAATCCTTGGGGCAT