| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073657 |
| Chromosome: | chromosome 7 |
| Location: | 6152001 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g356050 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGCAGGTGAAGGGCTGATGGCAAATGCGATGAGTTGAATTGTGCGT |
| Internal bar code: | TTGTATTCGTATGGTAATCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGATGGGGCTAAGCGTAG |
| Suggested primer 2: | CGACCGCTAGCTTAGACAGG |