Insertion junction: CLIP2.073659_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.073659
Chromosome:chromosome 14
Location:1052210
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre14.g614300 (1 of 1) 1.1.1.205 - IMP dehydrogenase / Inosinic acid dehydrogenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCTAATGCCTCCATGCCCACGCGAGACGCCGTCCCAGCGTCCCTACAT
Internal bar code:AGGATTTTTCACGGCACCAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:310
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGCTCAACTCCAATGTCT
Suggested primer 2:AGTAGGGGCTCCATGGATGT