Insertion junction: CLIP2.073665_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.073665
Chromosome:chromosome 10
Location:2354775
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g434700 (1 of 11) IPR017956 - AT hook, DNA-binding motif 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTACATATAGTAATTTGCCTCTGACGGGTCCGCTTGGCGAGTGGGTG
Internal bar code:CAGTAATTCGCATATTAAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1994
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CTATTACCACGTCCCCGTCG
Suggested primer 2:GGGCGCTCGAATTCCTATCA