| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073676 |
| Chromosome: | chromosome 14 |
| Location: | 1757575 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619200 | (1 of 140) IPR002048 - EF-hand domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTTGGGTAGGTCGCGTGGGTAGGTCAAGGGTGTCGCCCTTACACGCA |
| Internal bar code: | TTTTATAGCACGTTAACGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4402 |
| LEAP-Seq percent confirming: | 75.4491 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 167 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTCGATTGGTGATTGTC |
| Suggested primer 2: | GGCCGTGTTCGCATGTTATC |