| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.073680 |
| Chromosome: | chromosome 17 |
| Location: | 4714414 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732900 | (1 of 1) IPR000104//IPR001126 - Antifreeze protein, type I // UmuC domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAGGCGCCGCGCCAGCCGTCCCCGGCGCAGCAGCCGGTGGTCCCAGC |
| Internal bar code: | GATACGGTTGGTGAAGGCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATACACTGGGCGGGTCC |
| Suggested primer 2: | AGAGCTACACAACCACCAGC |