| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073682 |
| Chromosome: | chromosome 1 |
| Location: | 7604765 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053288 | (1 of 1) K01974 - RNA 3'-terminal phosphate cyclase (ATP) (RTCA, rtcA) | 5'UTR | |
| Cre01.g053300 | AIH1 | Agmatine iminohydrolase; (1 of 2) 3.5.3.12 - Agmatine deiminase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGTCCAAGCGAGTATATCAAGTGCGTTCCGATGATATAAAGTAGTG |
| Internal bar code: | GCACGTTCTCGTTTAGGCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGATTTGAAAGCGTGTGT |
| Suggested primer 2: | GAGAGGGAAACGGAAAGGGG |