| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073692 |
| Chromosome: | chromosome 6 |
| Location: | 1762655 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263050 | EFP2 | Mitochondrial elongation factor P; (1 of 1) PTHR30053:SF1 - ELONGATION FACTOR P (EF-P) FAMILY PROTEIN | 5'UTR |
| Cre06.g263100 | (1 of 2) PF10263 - SprT-like family (SprT-like) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTTGCGGAGTATTGAGCAGGTGCGAGACGATTAGGGCAATCGCGCAG |
| Internal bar code: | TGAACGGACCTGAGGTGCCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1674 |
| LEAP-Seq percent confirming: | 10.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGTGTTGGACAGCAGC |
| Suggested primer 2: | ATGTTCTGATCAGGTGCGCA |