Insertion junction: CLIP2.073700_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.073700
Chromosome:plastome
Location:2177
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802263 ChreCp001,2716989,petA (1 of 1) K02634 - apocytochrome f (petA); apocytochrome f precursor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAACAACCCTAACGTTGGTGGTTTCGGTCAGGCTGAAACTGAAATTGT
Internal bar code:AGTGGTCTTGGATCATGTATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:135
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGAGGCAGTGGAGGAAC
Suggested primer 2:AACCCACGTGAGGCTAATGG