| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073725 |
| Chromosome: | chromosome 6 |
| Location: | 2309379 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGTTATGTGTTGTTTTATGCGTGCAGATGCGCATGATTTCGTTCTGT |
| Internal bar code: | TTTGTGCCGTCTGGCGCCACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4200 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCAGCTCTGTCAAGGCC |
| Suggested primer 2: | GGCAAGGGTGTTGAGAGGTT |