| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073727 |
| Chromosome: | plastome |
| Location: | 8721 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802265 | ChreCp003,2716966,chlB | (1 of 1) K04039 - light-independent protochlorophyllide reductase subunit B (chlB); light-independent protochlorophyllide reductase subunit B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACGGCTACAGTGCAGGAGCCATCAAAATTTATTTACCCGAAGGCGAC |
| Internal bar code: | GATCCGATATTCGCCAGGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 13.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCAAAAATGGCTGCAGG |
| Suggested primer 2: | TAGCCCGTCTAGCAAGTTGC |