| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073731 |
| Chromosome: | chromosome 9 |
| Location: | 5865810 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411751 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGGGGCCCTGATCAGCGCACGGCAGCGCCCCTCAGCCGTGTCGTGTA |
| Internal bar code: | CGGGACATCGTTGGTAATCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1199 |
| LEAP-Seq percent confirming: | 42.1053 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGTATTGCGTCGCTCGG |
| Suggested primer 2: | ATGCACACTGTCGCAAACAC |