| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073743 |
| Chromosome: | chromosome 1 |
| Location: | 5268745 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036950 | CBA1 | (1 of 1) 2.7.8.26 - Adenosylcobinamide-GDP ribazoletransferase / Cobalamin-5'-phosphate synthase; Cobalamin 5'-phosphate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAACGTTTTCCTTGTGCTCTTTAACACATACACACACACACGTGCCCG |
| Internal bar code: | CGGTCTAACGTATTGCATTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATACTCGTCTTGCCCCGC |
| Suggested primer 2: | TCTACACACACGCACACTCC |