| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073744 |
| Chromosome: | chromosome 13 |
| Location: | 268802 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563733 | (1 of 1) K04368 - mitogen-activated protein kinase kinase 1 (MAP2K1, MEK1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTTTGCGTTGTTGGCCGTTACGGTTGGAGGCACCAAAGCGCAGCGGG |
| Internal bar code: | TTATATTACGTATTAGCCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAGGGGTACGTTTCGCCC |
| Suggested primer 2: | CGGGCCTCCTCTGCTTTAAA |