Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.073761 |
Chromosome: | chromosome 14 |
Location: | 3599728 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g630907 | APG5,ATG5 | (1 of 1) K08339 - autophagy-related protein 5 (ATG5); Autophagy-related protein | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACTTGAATTGATCAAGTTTAGGCGCCACGGTTTACGCAAACGGAAGC |
Internal bar code: | AAAATTATTGGGAACGGTGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2737 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 28 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTGTAATGTCGCGGGCATG |
Suggested primer 2: | GGCTCTTCGGACTCATGCTT |