| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073764 |
| Chromosome: | chromosome 7 |
| Location: | 1326329 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322450 | HLM13 | putative N-methyltransferase; (1 of 1) PF00855//PF00856 - PWWP domain (PWWP) // SET domain (SET) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGGCTTCCGCGCATGCAATCACCCTAAGATAAGACACTATCACGCGC |
| Internal bar code: | GCAGGGAAGGCCCAGGATTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1856 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAACCTGCGTGGCATATG |
| Suggested primer 2: | AAGCGAGAGACGAACAAGCA |