Insertion junction: CLIP2.073767_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.073767
Chromosome:plastome
Location:136800
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802320 chlN,2716986,ChreCp056 light-independent protochlorophyllide reductase subunit N; (1 of 1) K04038 - light-independent protochlorophyllide reductase subunit N (chlN) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGAAATTTTAGAATTAGTAACACGACCTCTTAGACGCAATCTAATGTC
Internal bar code:TTTTCTTCGTGAGCAAACATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:105
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGGGAAGCGAAAGGACA
Suggested primer 2:TTTCCAATAGGCCCCGATGG