Insertion junction: CLIP2.073770_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.073770
Chromosome:chromosome 3
Location:6471445
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g193550 mL46,MRPL46 (1 of 1) K17427 - large subunit ribosomal protein L46 (MRPL46); Mitochondrial ribosomal protein L46 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGGATGTTAGTACTGCCCTCGTTCTTAATACCTTACAGTGCACCTGA
Internal bar code:GTATATAATACGTAGATCCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1691
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:TTCATCGCGGTAGCTGATCC
Suggested primer 2:GTGCGACACGATCACGAATG