Insertion junction: CLIP2.073777_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.073777
Chromosome:chromosome 3
Location:4201990
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre03.g173550 (1 of 2) PTHR35100//PTHR35100:SF1 - FAMILY NOT NAMED // F15H11.13 PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGTCTTCCACAGCAGCTACGGCTCCTTCCGCGAGTGGGGCCGCCGACT
Internal bar code:AATTAGTCAGATTGACCGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:423
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGTTGACGAAGGAGACC
Suggested primer 2:TGTATGGGTATCGAGTGCGC