Insertion junction: CLIP2.073781_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.073781
Chromosome:chromosome 5
Location:3184601
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre05.g232100 (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACACACAACACACACACCCCAAATTGCACGCGCAATCCCTTAGTCCC
Internal bar code:ATAACCTTATTGGTCGTTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:382
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATTTCCGACAGCCCCGAATT
Suggested primer 2:GAAGGGCATCGACAAGGACA