Insertion junction: CLIP2.073783_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073783
Chromosome:chromosome 13
Location:2639058
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre13.g581150 NAT30 (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACAGTTGCGCCCCTTCAACAAACACACGGCGTCAAACCTCGCGCGGA
Internal bar code:ATCTAGCATTAAAGACAATCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:249
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCCACTTCCCTAGCTAGT
Suggested primer 2:GACTCTGGAGCGTGTGTTGA