| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073793 |
| Chromosome: | chromosome 1 |
| Location: | 5032103 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034900 | CDPK5 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1; Calcium/calmodulin-dependent protein kinase | 3'UTR |
| Cre01.g034950 | (1 of 1) K06184 - ATP-binding cassette, subfamily F, member 1 (ABCF1); Soluble ABC-F domain containing protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTGTGCACATTGTAATTCAGTAATTGTCATGCGTGTACCAGCCGAGG |
| Internal bar code: | TATAGGTTGTTCCGTTTTATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3454 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGTGAGCATATTGCCG |
| Suggested primer 2: | GTTGCTGACCTGCACTTGTG |