| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073798 |
| Chromosome: | chromosome 16 |
| Location: | 3308012 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666500 | BBS8 | (1 of 1) K16781 - tetratricopeptide repeat protein 8 (TTC8, BBS8); Bardet Biedl Syndrome-8 associated protein | 3'UTR |
| Cre16.g666550 | SOUL3 | SOUL heme-binding protein; (1 of 2) PTHR11220:SF35 - SOUL HEME-BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTAGCTGGGGGCAAGCGCTCCTGGCGCGCAGGAGATTTTAGGGCCCC |
| Internal bar code: | GGCATAGTTGCCGTTTGGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4272 |
| LEAP-Seq percent confirming: | 98.8889 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTATCAACCCAGCCACCT |
| Suggested primer 2: | TCCGCCTTGCTTCTTTTTGC |