Insertion junction: CLIP2.073800_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.073800
Chromosome:chromosome 14
Location:1483583
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g617500 FAP132,RSP14 Radial Spoke Protein 14; (1 of 1) PTHR15599:SF1 - RHABDOID TUMOR DELETION REGION PROTEIN 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAATCAAGTGTCACAAAGTACTGAGGTAGCAAACAGCGACCCACAGGCG
Internal bar code:TGTACTTTCATTCGACAATGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:368
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTAATGCCACGATTGCCG
Suggested primer 2:GCTGCTTCACTTGGTTGGTG