| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073805 |
| Chromosome: | chromosome 9 |
| Location: | 1303285 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400700 | 5'UTR | ||
| Cre09.g400750 | AMT5,AMT1Ea,AMT1E | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCGCCCCCATCCCGACCCCGAAGTTTGTCCTGAAAACAGCCATAAAT |
| Internal bar code: | CAAACTCTAAACAGTGCGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3000 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCCCCTCCTCCTCATCA |
| Suggested primer 2: | GGCTAATGCTTACTGTGCGC |