| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073808 |
| Chromosome: | chromosome 10 |
| Location: | 5621591 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGGGTAACTGGCTGAAGGCTGCGCCGAACCCCAGCAGCCCCGCACG |
| Internal bar code: | TGAAGGTACGGTATGTCCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1822 |
| LEAP-Seq percent confirming: | 98.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAATCCCAATGCGCATG |
| Suggested primer 2: | TATGAACAGCTGCTACGGGC |