Insertion junction: CLIP2.073811_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.073811
Chromosome:chromosome 3
Location:6848409
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g197550 TMEM48,NDC1 (1 of 1) K14315 - nucleoporin NDC1 (NDC1, TMEM48); Nuclear pore protein NDC1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGCTGGATGCTAACCCACCTAAACAGACACCGGCCAACCTCAACCGC
Internal bar code:CGCGAGTTATTGTTAAGGGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:1036
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGGAGTTCCTGGTTGTAA
Suggested primer 2:GTCGTTGGCCACAGTTCAAC