Insertion junction: CLIP2.073823_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.073823
Chromosome:chromosome 7
Location:2019445
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g800787 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGTCCGCTGATACTCCAGATTGGCACTACAGTACATTACTTATGTAG
Internal bar code:GAACTCTTCGCTGGTGCGAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2287
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCAACCGTGACACCATCC
Suggested primer 2:GTTTGGGTGAGTTCGCACAC