| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073825 |
| Chromosome: | chromosome 8 |
| Location: | 517767 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359400 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGCCGGGCGTCTTCACATCGCCCTTTCTCTTGTAAATAGGCGTCAGC |
| Internal bar code: | TAGCTTATATGGTTGATTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2096 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCTGTGTGTGCAGCTCG |
| Suggested primer 2: | AGTCCGGTAATGTGCAACGT |