| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073834 |
| Chromosome: | chromosome 8 |
| Location: | 2971945 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374900 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGCCCAGCGACCCTGGATCCGCGTGCGACAGATGCGCCTGACTTTCC |
| Internal bar code: | CTGACTGGCCCAATCAGATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3681 |
| LEAP-Seq percent confirming: | 87.037 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAACGGGCTCCAGAGAAC |
| Suggested primer 2: | ACATGGCGGGAGAACAAGAG |