Insertion junction: CLIP2.073849_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.073849
Chromosome:chromosome 3
Location:3190222
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g165100 PSAI1 (1 of 1) K02696 - photosystem I subunit VIII (psaI); Photosystem I reaction center subunit PsaI 5'UTR
Cre03.g165150 (1 of 31) IPR003594 - Histidine kinase-like ATPase, C-terminal domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATAACAAAACACGTACGCGCTTAGCATGTTGCGACGCTTAGGAACCAA
Internal bar code:AGCTTGTAAGTGGCTTGGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2185
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GACATGCCCTGACCTCTCAC
Suggested primer 2:ACGGCAGAACAAGGGAAGAG