| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073871 |
| Chromosome: | chromosome 4 |
| Location: | 3139020 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226001 | (1 of 1) IPR000104//IPR015943 - Antifreeze protein, type I // WD40/YVTN repeat-like-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGGGATGAACGTGGGCGGCTTGGGAGCGTGCGACTGTGAATGGGCTG |
| Internal bar code: | ACATTGTCACGTCAATTTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1234 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTTCTGCCAGCTACCTG |
| Suggested primer 2: | GTAGCAGTAACGCATTGCCG |