| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.073874 |
| Chromosome: | chromosome 6 |
| Location: | 8114025 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAGCGCTACCGATGGCCCAAGCAAGAGCCCTTAACTCATTAAGTGCA |
| Internal bar code: | AGCTGGGATGAGTTATTCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1755 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGACCATCCCAGCCAAG |
| Suggested primer 2: | ACTGTGTGTTGCTGGTGTGA |