| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.073887 |
| Chromosome: | chromosome 5 |
| Location: | 726929 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245450 | UMM4,COQ5A | (1 of 1) K06127 - 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (COQ5); Ubiquinone/menaquinone biosynthesis methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGTGCCTTACATCCAACGCCCAATCTGCTTCTCTGGATACCGCTCTTT |
| Internal bar code: | TTATTCAAACGTAGGGCCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGCTTGAAGCCTGGTA |
| Suggested primer 2: | TTGACAGATGATGCGCACGA |