| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073902 |
| Chromosome: | chromosome 17 |
| Location: | 3558676 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802069 | (1 of 1) PF16100 - RecQ-mediated genome instability protein 2 (RMI2) | 3'UTR | |
| lncRNA_TCONS_00163178 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTAATCAACGCTGCGCCTGATTCGGAGCAGGTCTTGCTGCTCGTAAA |
| Internal bar code: | TTTACCCATAAAGTTAAGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3004 |
| LEAP-Seq percent confirming: | 61.1111 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAAGACCAGGCAAGACT |
| Suggested primer 2: | AGTATGTCTTGCTGGTGGGC |