| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.073935 |
| Chromosome: | chromosome 14 |
| Location: | 1908656 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801622 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGTCCTGCGCAATGTCCTGCTGCACGCAACTTGTGCGCACGGGATCA |
| Internal bar code: | CTTGTTGTACTAGTGAGGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1350 |
| LEAP-Seq percent confirming: | 1.92308 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGATTGAGTGGTGGAGT |
| Suggested primer 2: | GTTGGGTTAGGGGGTGGATG |