Insertion junction: CLIP2.073937_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.073937
Chromosome:plastome
Location:21165
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802272 rpl36,2717053,secX,ChreCp010 (1 of 2) PF00444 - Ribosomal protein L36 (Ribosomal_L36); 50S ribosomal protein L36 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGGACGCTATTATTTACCAAAACAGGAAAAAGAATAGATTAGCTAATA
Internal bar code:GTTGTTGACGAGGTTGGCGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:236
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTTGTGGTGGCTTTGCTG
Suggested primer 2:GGTACCGCCACTGCCTATTT